Closures to a major road in Kingsholm for each of the last five years have left a business planning to leave the area due to lost earnings.
Resurfacing work is under way on Kingsholm Road as the area prepares for thousands of visitors to the Rugby World Cup in September.
The works, which began last week, are the latest in a long line of disruptions which have severely affected The Garage, in Kingsholm Road.
Benita McLean, owner of The Garage, said takings have halved over the past five years due to the continued works and it has led her to put her business up for sale.
She is calling for more access to be made available in nearby Denmark Road.
Benita said: "Kingsholm Road is closed from both sides and there's no access to my property. Three customers didn't turn up yesterday, and that's time I'll never get back.
"Over the years it's had a huge impact. But we can't get any compensation from anyone if we can't earn any money."
Major sewer works were carried out for six months in Kingsholm Road in 2012, which came after 11 month-long works in 2011 and seven month-long closures in 2010.
In 2013 the road was closed for three months, and last year it was closed for weeks while Severn Trent cleared a sewer pipe blockage near to the rugby club.
The Garage was forced to became an independent business in 2012 after losing a contract with Hyundai due to lost earnings because of the works.
Benita said: "The works have taken my business away from me, but no one is being held accountable.
"I absolutely understand that the council have to make the surface good. But we weren't notified that Denmark Road would be closed as well."
Severn Trent Water has previously said it tried to keep disruption to a minimum when they worked in the road.
Jason Humm, area highways manager for Gloucestershire County Council, said: "We wrote to residents and businesses at the beginning of February and no concerns were raised about this access.
"However, we appreciate the situation The Garage is in and we will speak to the workers on site to see if they can be as flexible as possible in allowing vehicles to access Kingsholm Road via Denmark Road wherever it is safe to do so."
